Journal: Breast Cancer Research : BCR

Article Title: Granulocytic immune infiltrates are essential for the efficient formation of breast cancer liver metastases

doi: 10.1186/s13058-015-0558-3

Figure Lengend Snippet: 4T1-derived breast cancer populations possess distinct and overlapping chemokine profiles. A heatmap representing the secreted levels of 25 chemokines measured using a chemokine array. The chemokines are grouped based on the immune cell types that they are known to recruit (multiple immune cell types, macrophages and/or lymphocytes, T lymphocytes and/or neutrophils, macrophages, T lymphocytes or neutrophils). Red color indicates chemokines that are highly expressed and the blue color denotes chemokines that are underexpressed.

Article Snippet: To detect and quantify chemokines secreted by each cell population, serum-free conditioned media (CM) was collected from cells cultured for 48 h and applied to a chemokine (mouse) quantitative antibody array (Abnova Corp., Taipai, Taiwan, cat#AA0118) following the manufacturer’s protocol.

Techniques: Derivative Assay

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin

doi: 10.1073/pnas.1616100114

Figure Lengend Snippet: PDZ1i inhibits key invasion proteins. (A) Metalloproteinase, (B) Cathepsin family, and ADAM9 expression. U1242 cells were treated with either DMSO or 50 μM PDZ1i for 2 h before radiation in serum-free media. After 48 h, media were collected and analyzed via a Proteome Profiler Human Protease Array Kit (R&D Systems). Relative protein amounts were quantified via ImageJ.

Article Snippet: After 48 h, media were collected and analyzed via a Proteome Profiler Human Protease Array Kit (R&D Systems).

Techniques: Expressing