Journal: Breast Cancer Research : BCR

Article Title: Granulocytic immune infiltrates are essential for the efficient formation of breast cancer liver metastases

doi: 10.1186/s13058-015-0558-3

Figure Lengend Snippet: 4T1-derived breast cancer populations possess distinct and overlapping chemokine profiles. A heatmap representing the secreted levels of 25 chemokines measured using a chemokine array. The chemokines are grouped based on the immune cell types that they are known to recruit (multiple immune cell types, macrophages and/or lymphocytes, T lymphocytes and/or neutrophils, macrophages, T lymphocytes or neutrophils). Red color indicates chemokines that are highly expressed and the blue color denotes chemokines that are underexpressed.

Article Snippet: To detect and quantify chemokines secreted by each cell population, serum-free conditioned media (CM) was collected from cells cultured for 48 h and applied to a chemokine (mouse) quantitative antibody array (Abnova Corp., Taipai, Taiwan, cat#AA0118) following the manufacturer’s protocol.

Techniques: Derivative Assay

Journal: Genes

Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep

doi: 10.3390/genes11040375

Figure Lengend Snippet: GDF9 promoter activity analysis in HEK293T. The g.46547859C>T , g.46548061A>G , and g.46548326C>T SNPs were in LD of Mongolia sheep populations, designated LD-M1. Luciferase reporter assay used the recombinant plasmids pGL3-1533, pGL3-1292-C, pGL3-726-A, pGL3-460-C, pGL3-177, and pGL3-110, and the g.46548326C>T , g.46548061A>G , and g.46547859C>T SNPs of LD-M1 is located between −1292 and −726, −726 and −460, and −460 and −177 bp upstream, respectively, of GDF9 translation initiation site. Luciferase activities of different GDF9 promoter fragments are shown on the right panel. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01.

Article Snippet: Firefly and Renilla luciferase activity levels were measured after 48 h transfection with a Dual Luciferase Report Array System (Promega, Madison, WI, USA) in a Varisskan Flash Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers’ protocols.

Techniques: Activity Assay, Luciferase, Reporter Assay, Recombinant

Journal: Genes

Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep

doi: 10.3390/genes11040375

Figure Lengend Snippet: Effect of the LD-M1 on promoter activity of ovine GDF9 . ( a ) Luciferase activity results showed that the C allele at the g.46547859C>T SNP could decrease promoter activity than T allele. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01. ( b ) Prediction of transcription factor binding motif in conserved sequence including the g.46547859C>T SNP site.

Article Snippet: Firefly and Renilla luciferase activity levels were measured after 48 h transfection with a Dual Luciferase Report Array System (Promega, Madison, WI, USA) in a Varisskan Flash Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers’ protocols.

Techniques: Activity Assay, Luciferase, Binding Assay, Sequencing